Affinity chromatography is established and developed based on the principle of affinity adsorption between biomolecules and other ligand molecules, such as antigen and antibody, enzyme and substrate, hormone and receptor, complementary strands in nucleic acids, polysaccharide and protein complexes, etc. Affinity chromatography achieves the purpose of purifying target molecules through specific adsorption and dissociation between ligands on the medium and target molecules. Due to this specific force, affinity chromatography has characteristics such as high selectivity and high activity recovery.
Used for the separation and purification of biological macromolecules, such as PEG-protein, VLPs, and viruses.
His-tagged protein purification affinity chromatography resin
Product | Protein/chelation content/1ml | Particle size μm | Flow Rate cm/h | Pressure MPa | pH | Applications |
Ni FF (IDA) | ≥30mg His-tagged protein | 45-165 | ≥300 | ≤0.3 | 2-14(short) 3-12(long) | Purify His-tagged protein |
Ni FF (IMAC) | ≥40mg His-tagged protein | Large-scale purification of His-tagged proteins |
Ni FF (TED) | ≥10mg His-tagged protein | It can withstand 100mM EDTA and 10mM DTT, and can be thoroughly cleaned with 1M NaOH directly without nickel removal. |
GST-tagged protein purification affinity chromatography resin
Product | Capacity/1ml |
Particle size μm |
Flow Rate cm/h |
Pressure MPa | pH | Applications |
GST FF | ≥20mg GST-tagged protein | 45-165 | ≥250 | ≤0.3 | 3-12(long) | Purify GST-tagged protein |
Antibody purification affinity chromatography resin
Product | Capacity/1ml |
Particle size μm |
Flow Rate cm/h |
Pressure MPa | pH | Applications |
arProtein A HR | ≥50 IgG | 45-165 | ≥700 | ≤0.5 |
2-10(short) 3-9(long)
| Purified antibody, immunoglobulin, and Fc fusion protein |
Protein G 4FF | 20-30 IgG | ≥250 | ≤0.3 |
Purification affinity resin for serine protease
Product | Capacity/1ml |
Particle size μm |
Flow Rate cm/h |
Pressure MPa | pH | Applications |
Benzamidine FF(LS) | 10-20mg trypsin | 45-165 | ≥300 | ≤0.3 |
1-9(short) 2-8(long)
| Separation and purification of trypsin, chymotrypsin, elastase, etc |
Benzamidine 4FF(HS) | ≥30mg trypsin | ≥250 | ≤0.3 |
Virus, virus/microbial antigen purification affinity resin
Product | Lysozyme loading mg/ml |
Particle size μm |
Flow Rate cm/h |
Pressure MPa | pH | Storage solution/temperature |
PS HPL | ≥3mg/ml | 45-165 | ≥300 | ≤0.3 |
5-12(short) 5-12(long) | 20% ethanol/4-30℃ |
Plasmid purification affinity resin
Product | Capacity mg/ml |
Particle size μm |
Flow Rate cm/h |
Pressure MPa | pH | Applications |
Plasmid HPR | ≥2mg（pDNA）/ml | 25-45 | ≥150 | ≤0.5 |
2-13(short) 3-11(long) | Separation and purification of pDNA |
