Gel filtration chromatography separates molecules in the liquid mobile phase based on their physical sizes. Molecules with diameters larger than the pore size of the packing material cannot enter the pores and are completely excluded, passing quickly through the column and eluting first. In order of molecular weight, smaller molecules are more able to enter the pores of the packing material and elute later, thus achieving separation. Gel filtration chromatography is also known as size exclusion chromatography or molecular sieve chromatography.

Application strategy of gel filtration chromatography medium

● Gel filtration chromatography is commonly used for subsequent purification stages with fewer impurities.

● Gel filtration chromatography is used for the purification of samples with small volumes.

● It can also be used in the rough purification stage during separation (such as desalination).

● Only one type of buffer is required for gel filtration chromatography separation, and the type of buffer has little effect on the separation efficiency. Adding 150 mM sodium chloride to the buffer can effectively reduce the nonspecific adsorption of the target protein.